Related Experiment Video

Updated: Jan 7, 2026

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues
08:06

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Published on: July 8, 2020

6.0K

RLX-201, a Novel mTORC1 Inhibitor With Potential to Promote Skin Longevity and Cellular Health

Manpreet Randhawa1, Krzysztof Bojanowski2, Teresa Ferraro3

  • 1Rapalogix Health Inc., New York, New York.

Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [Et Al.]
|December 17, 2025
PubMed
Abstract

Insights

Targeting mechanistic target of rapamycin complex 1 (mTORC1) with RLX-201 selectively inhibits aging-related fibroblast hyperactivity, preserving mTORC2 and promoting skin longevity.

Area of Science:

  • Cellular senescence
  • Aging research
  • Dermatology

Background:

  • Mechanistic target of rapamycin complex 1 (mTORC1) hyperactivity drives aging phenotypes, including extracellular matrix degradation.
  • Targeting mTORC1 selectively offers a strategy for promoting skin longevity.

Purpose of the Study:

  • Assess age-related mTORC1 activity in fibroblasts.
  • Evaluate the novel mTOR inhibitor RLX-201 for its effects on mTORC1 and mTORC2 in skin fibroblasts.

Main Methods:

  • Human dermal fibroblasts from young and older donors analyzed for phosphorylated RPS6 (p-RPS6).
  • RLX-201's impact on mTORC1 (p70S6K) and mTORC2 (pAKT Ser473) assessed via HTRF assays.

Main Results:

  • Older fibroblasts exhibited elevated basal mTORC1 activity (higher p-RPS6).
  • RLX-201 selectively inhibited mTORC1 without affecting mTORC2, unlike rapamycin.
  • RLX-201 treatment shifted fibroblast morphology towards a younger phenotype.

Conclusions:

  • mTORC1 hyperactivity is a hallmark of aging fibroblasts, contributing to skin aging.
  • RLX-201 selectively inhibits mTORC1, preserving mTORC2, and may counteract skin aging.
  • RLX-201 rejuvenates fibroblast function, supporting its potential for skin longevity.

More Related Videos

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

18.5K
Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ10:05

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Published on: May 8, 2020

2.2K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.6K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Liver Regeneration01:24

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
4.2K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K