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Sevoflurane Postconditioning Regulates Mitophagy Through the PINK1/Parkin Pathway to Alleviate Myocardial

Yanming Xue1, Liqun Jia2, Jianzhong Zhang1

  • 1Department of Anesthesiology, Yantaishan Hospital Affiliated to Binzhou Medical University, Yantai, Shandong, China.

Chemical Biology & Drug Design
|December 17, 2025
PubMed

Insights

Sevoflurane postconditioning protects the heart from reperfusion injury by inhibiting excessive mitophagy through the PINK1/Parkin pathway. This intervention improves microvascular function and reduces infarct size in myocardial ischemia/reperfusion injury.

Area of Science:

  • Cardiology
  • Cell Biology
  • Pharmacology

Background:

  • Myocardial ischemia/reperfusion (I/R) injury causes significant microcirculatory damage.
  • Mitophagy, the selective degradation of mitochondria, plays a role in I/R injury.
  • The PTEN-induced kinase 1 (PINK1)/Parkin pathway regulates mitophagy.

Purpose of the Study:

  • To investigate if sevoflurane postconditioning (SpostC) alleviates myocardial microcirculatory reperfusion injury.
  • To determine if SpostC regulates mitophagy via the PINK1/Parkin pathway.

Main Methods:

  • Established a mouse model of myocardial I/R injury.
  • Assessed microvascular perfusion, vascular pathology, and infarct size.
  • Analyzed protein expression of key mitophagy and endothelial markers using Western blotting.
  • Utilized in vitro models (hCMECs subjected to OGD/R) to assess cell viability, barrier function, and mitophagy.

Main Results:

  • SpostC enhanced microvascular perfusion and reduced infarct size.
  • SpostC suppressed excessive mitophagy and restored endothelial function.
  • Inhibiting mitophagy (using CCCP) or overexpressing PINK1 reversed the protective effects of SpostC.

Conclusions:

  • SpostC exerts protective effects against myocardial microcirculatory reperfusion injury.
  • These protective effects are mediated by the inhibition of the PINK1/Parkin-mediated mitophagy pathway.
Keywords:
PINK1/Parkinendothelial dysfunctionmitophagymyocardial microcirculatory reperfusion injurysevoflurane postconditioning

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