Screening and characterization of ovalbumin active peptides for muscle repair: Computer simulation and in vitro
Yuqing Lei1, Jiaying Huo1, Shijun Li1
1Engineering Research Center of Bio-process, Ministry of Education/Key Laboratory for Agricultural Products Processing of Anhui Province/ School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China.
Abstract:
Ovalbumin (OVA) has demonstrated significant potential in promoting muscle repair due to its rich amino acid composition. Previous findings have confirmed the muscle repair-promoting effects of OVA. In this study, a total of five peptides with muscle repair activity were identified through in vitro simulated digestion, multi-stage chromatography purification, and computer-based virtual screening. Repair effects were then validated using a C2C12 cell model. Experimental results showed that SK-10 had significant effect, increasing the viability of dexamethasone (DEX)-induced C2C12 cells (from 73.33 % to 85.15 %) and restoring the levels of inflammatory factors (IL-6, IL-1β, TNF-α) to normal levels. Further qRT-PCR (quantitative real-time polymerase chain reaction) and Western blot (WB) experiments indicated that SK-10 could activate the PI3K-Akt-mTOR signaling pathway, thereby promoting protein synthesis and ultimately enhancing the proliferative capacity of myoblasts. The study could provide a theoretical basis for promoting muscle repair by OVA active peptide.
