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Wheat flour tortilla authenticity verification using targeted elemental profiling-based multifunction classification

Uriel Arellano1, Ilse María Hernández-Romero1, Lucy T González1

  • 1Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, N.L., Mexico.

Food Chemistry
|December 17, 2025
PubMed
Summary
This summary is machine-generated.

This study used elemental analysis to authenticate wheat flour tortillas, identifying geographic origin and manufacturing methods. Decision tree models achieved 100% accuracy, offering a reliable framework for tortilla authentication.

Keywords:
Elemental compositionFood authenticationGeographical originManufacturing processMultifunction classificationWheat flour tortillas

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Chemometrics

Background:

  • Wheat flour tortillas are a Mexican staple with limited authentication methods.
  • Reliable methods are needed for quality control and consumer confidence.

Purpose of the Study:

  • To develop and evaluate data-driven methods for authenticating wheat flour tortillas.
  • To determine geographic origin and manufacturing processes using elemental profiling.

Main Methods:

  • Analysis of 20-element profiles in 145 tortilla samples.
  • Data preprocessing including autoscaling and mean centering.
  • Application of principal component analysis, linear discriminant analysis, k-nearest neighbors, and decision tree models.

Main Results:

  • Geographic origin significantly influenced elemental composition more than the manufacturing process.
  • Decision tree models demonstrated 100% sensitivity, specificity, and accuracy for multifunction classification.
  • K-nearest neighbors and linear discriminant analysis showed high accuracies (88-100% and 83-92%, respectively).

Conclusions:

  • A robust, data-driven framework for tortilla authentication was established.
  • The findings support enhanced quality control, regulatory oversight, and consumer trust in tortilla products.
  • Elemental profiling combined with chemometric models offers a powerful tool for food authentication.