A simple, efficient, and rapid pipette tip-integrated silanized melamine sponge device for UPLC-MS/MS analysis of...

In this study, a facile and efficient pipette tip-integrated silanized melamine sponge device was developed for the rapid quantification of multi-pesticide residues in cowpea by ultra-performance liquid chromatography-tandem mass spectrometry. By embedding silanized melamine sponges within a pipette tip architecture, the device facilitates efficient matrix cleanup through simple aspiration-dispensing maneuvers, substantially simplifying sample pretreatment workflows. Comparative evaluations against conventional commercial adsorbents revealed that the proposed system exhibits comparable or enhanced purification performance. Method validation demonstrated that target analytes achieved recoveries spanning 81.0 %-115.4 % across three spiking concentrations, with intra- and inter-day precisions both below 15 %. Additionally, excellent linearity (R²≥0.9985), negligible matrix effects (-18.3 % to 19.8 %), and low limits of detection (0.025-2.40 μg·kg⁻¹) and quantification (0.05-5.00 μg·kg⁻¹) were attained. These findings underscore the considerable potential of the developed purification device for pesticide multi-residue analysis in complex food matrices.