Multi-amplified electrochemical aptasensor for florfenicol detection based on functionalized trimetallic MOF-derived...

Florfenicol (FF) is a broad-spectrum antibiotic, and its residues in food pose health risks, including immune and organ toxicity. Therefore, the development of efficient detection methods is of significant importance. In this study, an electrochemical aptasensor based on a multi-signal amplification strategy was constructed, integrating trimetallic MOF-derived Zn/CoMnN-doped porous carbon (AuNPs@Zn/CoMnN-PCs) with DNAzyme-mediated hybridization chain reaction (HCR) cascade amplification. This combination enhances the interfacial conductivity and significantly improves detection sensitivity and specificity. The aptasensor exhibited a good linear response within the concentration range of 1.0 × 10^-5 to 1.0 × 10³ ng·mL^-1, with a detection limit as low as 1.97 × 10^-6 ng·mL^-1. Recoveries of spiked milk samples ranged from 96.4 % to 102.3 %. The results demonstrate that this enzyme-free electrochemical platform holds great potential for FF residue monitoring and related food safety analysis, providing a reference for the application of multi-signal amplification aptasensors in the detection of other antibiotics.