Genome-wide characterization and expression pattern analysis of Xanthoceras sorbifolium FAD at different growth...

Xanthoceras sorbifolium is a crucial oil tree species in northern China. Additionally, fatty acid constituents in its fruits possess remarkable medicinal value. Hence, probing the regulatory role of FAD family members in fatty acid components accumulation in X. sorbifolium fruits is crucial. Drawing on X. sorbifolium genome data, 22 XsFAD members distributed across 10 chromosomes were identified. The physicochemical properties and subcellular localization predictions thereof were analyzed. Phylogenetic and conserved domain analyses demonstrated that same subfamily members are highly conserved in both gene structure and conserved domains. Cis-acting element analysis indicated that XsFAD members are highly susceptible to light. Furthermore, transcriptome data and qRT-PCR findings corroborated that XsFAD members play an active regulatory role in X. sorbifolium fruit ripening and fatty acid metabolism. The bioinformatic analysis and preliminary verification of regulatory functions of XsFAD members establish conditions for in-depth research on the functions of key XsFAD in the future. The study findings will be conducive to determine the accumulation of fatty acids substances in X. sorbifolium and screening of germplasm resources with superior traits.