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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Related Experiment Video

Updated: May 4, 2026

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
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MicroRNA-127-3p Inhibits In Vitro Osteogenesis and Dampens Trauma-Induced Heterotopic Ossification In Vivo.

Victor Gustavo Balera Brito1,2, Austin Bell-Hensley3, Hongjun Zheng1

  • 1Department of Orthopaedic Surgery, Washington University School of Medicine, St. Louis, Missouri, USA.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|December 18, 2025
PubMed
Summary

Overexpressing miR-127-3p inhibits bone formation and reduces ectopic bone growth. This microRNA

Keywords:
heterotopic ossificationmiR‐127‐3pmicroRNAnanoparticle deliveryosteogenesis

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Area of Science:

  • Molecular Biology
  • Skeletal Biology
  • RNA Biology

Background:

  • MicroRNAs regulate cellular pathways and are crucial for skeletal development.
  • Elevated miR-127-3p levels are observed in osteoporotic patients and ovariectomized mice.
  • Previous studies suggest a role for miR-127-3p in bone formation regulation.

Purpose of the Study:

  • To investigate the function of miR-127-3p in osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells (hBMSCs).
  • To assess the therapeutic potential of miR-127-3p in treating heterotopic ossification.

Main Methods:

  • Overexpression and inhibition of miR-127-3p in hBMSCs.
  • Bulk RNA-sequencing to identify affected cellular pathways.
  • In vivo studies using a mouse model of heterotopic ossification with local delivery of miR-127-3p mimics via nanoparticle technology.

Main Results:

  • miR-127-3p mimic overexpression robustly inhibited in vitro osteogenesis and cell proliferation.
  • RNA-sequencing revealed suppression of proliferation-related pathways.
  • Local delivery of miR-127-3p mimics significantly reduced ectopic bone formation in a mouse model.

Conclusions:

  • miR-127-3p acts as a negative regulator of osteogenic differentiation.
  • Overexpression of miR-127-3p suppresses bone formation and may be a therapeutic strategy for heterotopic ossification.