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Toward Accurate Real-Time Bioaerosol Monitoring in the Particle Size Range 1 μm-70 μm.

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Summary

A new method evaluates automated bioaerosol monitors, crucial for real-time pollen and fungal spore data. This ensures accurate air quality measurements, vital for public health and climate research.

Keywords:
algorithmautomated bioaerosol monitorsbioaerosolcalibrationclassification ratecorrection factorcounting efficiencyfluorescenceholographymachine learningmeasurement efficiencymonitoringpolenopollen

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Area of Science:

  • Environmental science
  • Atmospheric science
  • Biotechnology

Background:

  • Automated bioaerosol monitors offer real-time data on airborne particles like pollen and fungal spores, impacting health and climate.
  • Current technologies require thorough characterization and performance evaluation due to their novelty.

Purpose of the Study:

  • To develop a general method for evaluating the hardware and software performance of automated bioaerosol monitors.
  • To establish a traceable framework for accurate real-time bioaerosol measurements.

Main Methods:

  • Combined custom optical particle counter, inkjet aerosol generator (IAG), and particle tracking velocimetry (PTV) for particle sizing up to 70 μm.
  • Determined size-dependent counting efficiency and unit-to-unit variability of five bioaerosol monitors.
  • Assessed machine learning (ML) classification efficiency using controlled pollen samples and quantified influencing factors.

Main Results:

  • Established a traceable method for evaluating bioaerosol monitor performance across the pollen and fungal spore size range.
  • Quantified the classification efficiency of the MeteoSwiss ML algorithm and identified factors affecting it.
  • Demonstrated the capability for accurate measurements of large, low-concentration bioaerosols.

Conclusions:

  • The developed method provides a reliable framework for validating automated bioaerosol monitors.
  • Findings will improve ML algorithm training and ensure data accuracy comparable to legislated air quality measurements.
  • This work is a key step towards integrating real-time bioaerosol monitoring into European legislation.