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Related Experiment Video

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Ex Situ Normothermic Machine Perfusion of Donor Livers

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Machine Perfusion Parameters to Predict Graft Injury or Early Function After Liver Transplantation.

Charlotte von Horn¹, Nurith Winzen, Laura Malkus

¹From the Department for Surgical Research, University Hospital Essen, Germany.

Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation

|December 18, 2025

Summary

This summary is machine-generated.

Machine perfusion biomarkers like aspartate aminotransferase and lactate can predict liver graft injury, but not early functional recovery. Further research into functional assessments during perfusion is recommended.

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Area of Science:

Organ transplantation
Graft viability assessment
Machine perfusion technology

Background:

Acceptance criteria for donor organs are limited.
Surgeons need tools to estimate graft quality.
Biomarkers during machine perfusion may predict post-transplant graft fate.

Purpose of the Study:

Evaluate biomarkers during machine perfusion.
Predict graft fate after transplantation.
Assess morphological injury and early functional recovery.

Main Methods:

Twenty extended criteria donor livers were machine perfused.
Machine perfusion solution (MPS) was used.
Liver function data were collected after 90 minutes.

Main Results:

Perfusate glucose, aspartate aminotransferase, pH, and lactate correlated with peak transaminase levels post-transplant.
The product of aspartate aminotransferase and lactate showed the best correlation (r² = 0.86).
Early liver function did not significantly correlate with these parameters.

Conclusions:

Aspartate aminotransferase level during perfusion was the best prognostic factor (r² = 0.11).
Hepatic functional recovery did not correlate with morphological reperfusion injury (r² = 0.15).
Functional investigations during machine perfusion require further development.