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Related Experiment Video

Updated: Jan 8, 2026

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

A simple method to efficiently generate structural variation in plants.

Lindsey L Bechen¹, Naiyara Ahsan^2,3, Alefiyah Bahrainwala²

¹Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of America.

|December 18, 2025

Summary

This summary is machine-generated.

A new method uses etoposide to induce structural variation in plants, creating new traits. This simple technique generates heritable genetic changes and altered phenotypes in Arabidopsis thaliana, offering an alternative to irradiation.

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Area of Science:

Plant genetics and breeding
Genomics
Molecular biology

Background:

Phenotypic variation is crucial for selecting desirable traits in plants.
Structural variants (SVs) like deletions and inversions have significant phenotypic impact.
Pan-genome studies underscore the role of SVs in plant evolution and trait selection.

Purpose of the Study:

To introduce a simple and efficient method for inducing structural variation in plants.
To demonstrate the efficacy of etoposide in generating heritable SVs and altered phenotypes.
To provide a resource-efficient alternative to irradiation for inducing large-effect SVs.

Main Methods:

Treatment of Arabidopsis thaliana with the topoisomerase II inhibitor etoposide.
Induction of structural variation through a short growth period.
Long-read sequencing and genetic analyses to identify causative variants.

Main Results:

Etoposide treatment induced high-frequency, heritable structural variation in Arabidopsis thaliana.
Deletions and inversions were identified as the genetic basis for observed semi-dominant and recessive phenotypes.
The method proved effective in generating altered phenotypes linked to SVs.

Conclusions:

A simple etoposide-based method effectively induces heritable structural variation and phenotypes in plants.
This approach offers a low-resource, broadly applicable alternative to irradiation for plant breeding.
The technique facilitates the study and selection of novel traits driven by structural variation.