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Updated: May 5, 2026
Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
Published on: August 2, 2011
Research note: Development of a real-time recombinase polymerase amplification assay for the detection of goose
Su Lin1, Xiuqin Chen1, Xiaofei Lin2
1Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou 350003, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou 350013, China.
Abstract:
Goose parvovirus (GPV) remains a major pathogen in waterfowl production, threatening animal health and industry sustainability. In this study, a real-time recombinase polymerase amplification (RPA) assay was developed for rapid and specific detection of GPV. The assay employed a primer‒probe system targeting the VP1 gene and operated under isothermal conditions at 40°C. Analytical sensitivity was determined using serial dilutions of plasmid DNA, establishing a detection limit of 3.0 × 10¹ copies/μL with high linearity (R² = 0.99). No cross-reactivity was observed with other common avian pathogens, confirming the specificity of the assay. Clinical validation using 53 field samples demonstrated 98.1 % overall agreement with real-time qPCR. This method requires minimal equipment and yields results within 20 min, making it more suitable for field applications than conventional PCR- or qPCR-based platforms. These findings indicate that the developed real-time RPA assay is a rapid, sensitive, and reliable diagnostic tool for GPV detection, with potential utility in epidemiological surveillance and disease control in waterfowl populations.
