Signal and Time Resolved Information Experiment (SATIRE): An NMR Supersequence for Monitoring Complex Environmental...

Area of Science:

• Nuclear Magnetic Resonance (NMR) Spectroscopy
• Analytical Chemistry
• Biophysical Chemistry

Background:

• Complex systems monitoring demands both spectral dispersion (2D NMR) and high temporal resolution (1D ¹H NMR).
• Conventional interleaved 1D and 2D NMR experiments create data gaps, limiting temporal resolution and efficiency.
• Low signal-to-noise ratio (SNR) in complex samples necessitates long acquisition times, hindering dynamic process monitoring.

Purpose of the Study:

• To present the Signal And TIme Resolved information Experiment (SATIRE), a novel NMR supersequence for simultaneous 1D and 2D data acquisition.
• To enable efficient monitoring of complex systems with low SNR by minimizing experimental time.
• To demonstrate the capability of SATIRE for real-time process monitoring without compromising data quality.

Main Methods:

• SATIRE utilizes leftover ¹H magnetization from HSQC experiments to acquire 1D ¹H NMR data within the same scan.
• The method employs a single receiver for simultaneous data collection, creating a pseudo-3D data set.
• Experiments were validated using benchtop NMR for sucrose hydrolysis and high-field NMR (500 MHz) for *in vivo* anoxic stress monitoring.

Main Results:

• SATIRE achieves simultaneous acquisition of 1D ¹H and 2D HSQC NMR data without compromising SNR or spectral quality.
• The method effectively eliminates time gaps inherent in conventional interleaved experiments.
• Demonstrated successful application in monitoring sucrose hydrolysis and *in vivo* anoxic stress, showcasing its utility for complex systems.

Conclusions:

• SATIRE offers a significant advancement in NMR methodology, enabling simultaneous 1D and 2D data acquisition.
• The technique enhances the efficiency and effectiveness of monitoring complex dynamic processes, particularly those with low SNR.
• SATIRE supports quantitative analysis and time-resolved studies across diverse applications at natural abundance.