Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transcription01:10

Transcription

154.8K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
154.8K
Transgenic Plants02:50

Transgenic Plants

8.4K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.4K
Morphogenesis02:19

Morphogenesis

30.1K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
30.1K
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

402
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
402

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk of neuropsychological impairment among therapeutic community residents: relationship with dropout and spontaneous recovery during treatment.

BMC psychiatry·2026
Same author

Genome-wide screen for deficiencies modifying Cyclin G-induced developmental instability in Drosophila melanogaster.

Genetics·2026
Same author

Author Correction: Heterochromatin boundaries maintain centromere position, size and number.

Nature structural & molecular biology·2026
Same author

Bacterial chromatin remodeling associated with transcription-induced domains at pathogenicity Islands.

Nature communications·2026
Same author

Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesis.

The EMBO journal·2026
Same author

Linker histones enhance robustness in diurnal transcription dynamics.

Quantitative plant biology·2025

Related Experiment Video

Updated: Jan 8, 2026

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

10.1K

Genome-scale transcriptome augmentation during Arabidopsis thaliana photomorphogenesis.

Geoffrey Schivre1,2, Léa Wolff1, Filippo Maria Mirasole3

  • 1Institut de biologie de l'École normale supérieure (IBENS), École normale supérieure, CNRS, INSERM, PSL Université, Paris, 75005, France.

Nature Communications
|December 18, 2025
PubMed
Summary

Light triggers massive gene upregulation during plant photomorphogenesis, increasing cellular transcripts twofold. This study reconciles transcriptome dynamics with epigenomic data, revealing light

More Related Videos

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
10:10

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

16.2K
Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
09:41

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

12.8K

Related Experiment Videos

Last Updated: Jan 8, 2026

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

10.1K
Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
10:10

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

16.2K
Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
09:41

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

12.8K

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Plant photomorphogenesis involves light-induced gene expression changes and increased RNA Polymerase II activity.
  • Previous transcriptome analyses did not fully explain the observed gene expression dynamics during this transition.
  • A discrepancy existed between transcriptomic data and epigenomic/cytogenetic observations in light-regulated development.

Purpose of the Study:

  • To reconcile transcriptome dynamics with epigenomic and cytogenetic data during Arabidopsis thaliana cotyledon photomorphogenesis.
  • To investigate the global impact of light on gene expression and cellular transcript levels.
  • To re-evaluate the role of light-induced transcription factors in gene regulation.

Main Methods:

  • Utilized a spike-in RNA-sequencing (RNA-seq) experimental and bioinformatic pipeline.
  • Applied re-normalization of spike-free RNA-seq datasets using stable endogenous transcript levels.
  • Analyzed epigenomic and cytogenetic data alongside transcriptomic profiles.

Main Results:

  • Identified a rapid, light-induced upregulation of 94% of differentially expressed genes within six hours.
  • Observed a two-fold increase in cellular transcript levels during the photomorphogenesis transition.
  • Demonstrated that key light-induced transcription factors, like ELONGATED HYPOCOTYL 5 (HY5), have a predominantly positive effect on target genes.

Conclusions:

  • Provides a paradigm shift in understanding light-mediated global genome regulation in plants.
  • Highlights the importance of accurate transcriptome quantification for interpreting developmental transitions.
  • Opens new avenues for studying transcriptome size control in various plant developmental and environmental responses.