Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

5.1K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

10.3K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.3K
The Nucleolus02:55

The Nucleolus

10.2K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
10.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

4.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.6K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

8.2K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.2K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

6.9K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiscale in vivo imaging of tumor evolution using a germline conditional triple-reporter mouse.

Nature biotechnology·2026
Same author

Malignant T Cells Operate at the Edge of Redox Tolerance and Propagate Oxidative Stress in Cutaneous T-Cell Lymphoma.

The Journal of investigative dermatology·2026
Same author

iMUT-seq mapping of DSB-induced mutations with high sensitivity at single-nucleotide resolution.

Nature protocols·2026
Same author

Fibroblastic aspartoacylase suppresses TGFβ-mediated responses and cancer progression.

Nature communications·2026
Same author

Integrated stress response couples mitochondrial fitness with lineage reprogramming to drive cancer evolution.

Nature cell biology·2026
Same author

Coordinated regulation of mRNA translation and stability by ZC3H7A and ZC3H7B RNA-binding proteins.

Cell reports·2026

Related Experiment Video

Updated: Jan 8, 2026

Efficient Gene Knockdown in the Liver via Intrasplenic Injection of Adeno-Associated Virus Serotype 8 (AAV8)-Delivered Small Hairpin RNA
04:29

Efficient Gene Knockdown in the Liver via Intrasplenic Injection of Adeno-Associated Virus Serotype 8 (AAV8)-Delivered Small Hairpin RNA

Published on: November 1, 2024

806

Nucleophosmin supports WNT-driven hyperproliferation and tumor initiation.

Georgios Kanellos1, Chiara Giacomelli2, Alexander Raven2

  • 1Cancer Research UK Scotland Institute, Glasgow, UK. g.kanellos@crukscotlandinstitute.ac.uk.

Nature Genetics
|December 18, 2025
PubMed
Summary

Nucleophosmin (NPM1) supports WNT-driven cancer by suppressing stress responses. Inhibiting NPM1 may be a therapeutic strategy for WNT-driven tumors, especially in colorectal cancer.

More Related Videos

The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

113.5K
Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens
09:33

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

1.6K

Related Experiment Videos

Last Updated: Jan 8, 2026

Efficient Gene Knockdown in the Liver via Intrasplenic Injection of Adeno-Associated Virus Serotype 8 (AAV8)-Delivered Small Hairpin RNA
04:29

Efficient Gene Knockdown in the Liver via Intrasplenic Injection of Adeno-Associated Virus Serotype 8 (AAV8)-Delivered Small Hairpin RNA

Published on: November 1, 2024

806
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

113.5K
Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens
09:33

Author Spotlight: Finding New Therapeutic Targets for Malignant Peripheral Nerve Sheath Tumor Through Genome-Scale shRNA Screens

Published on: August 25, 2023

1.6K

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • Nucleophosmin (NPM1) is a nucleolar protein implicated in hematopoietic malignancies and overexpressed in solid tumors.
  • Its functional role in solid tumor progression, particularly in WNT-driven cancers, remains unclear.

Purpose of the Study:

  • To investigate the role of NPM1 in WNT-driven intestinal and liver tumorigenesis.
  • To elucidate the molecular mechanisms by which NPM1 influences cancer progression and the integrated stress response.

Main Methods:

  • Studied NPM1 expression in WNT-responsive tissues following APC loss.
  • Analyzed the impact of NPM1 loss on protein synthesis, ribosome function, and p53 activation.
  • Correlated NPM1 expression and deletion with WNT signaling, proliferation, and TP53 status in human colorectal cancer (CRC).

Main Results:

  • NPM1 is upregulated after APC loss and promotes WNT-driven tumorigenesis.
  • NPM1 loss triggers ribosome pausing, integrated stress response, and p53 activation, mediating an antitumorigenic effect.
  • NPM1 expression correlates with WNT signaling and proliferation in CRC; NPM1 deletions are linked to TP53 inactivation.

Conclusions:

  • NPM1 is a critical WNT effector that sustains hyperproliferation and tumorigenesis by attenuating the integrated stress response and p53 activation.
  • NPM1 is dispensable for adult epithelial homeostasis, making it a potential therapeutic target in p53-proficient WNT-driven tumors like refractory KRAS-mutant CRC and hepatic cancers.