Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hybrid solid-liquid optics enable scalable, high-resolution light-sheet microscopy across diverse immersion media.

Nature biotechnology·2026

Same author

Dendrobium officinale leaf extract extends the mean lifespan in Caenorhabditis elegans via the DAF-16/SOD-3 axis.

Biogerontology·2026

Same author

Chiral Molecular Intercalation Enables Light-Controlled 2D Multiferroic Heterostructures.

Nano letters·2026

Same author

A chromosome-level genome assembly of the waterlily aphid Rhopalosiphum nymphaeae Linnaeus.

Scientific data·2026

Same author

Meta-analysis of the skin redraping method and the Z-plasty method in the correction of epicanthus.

Medicine·2026

Same author

Integration of Multi-Omics Data Identifies the Role of the Selenium-Related Gene PNPO and Pre-exhausted CD127<sup>-</sup> CD8<sup>+</sup> T Cells in Laryngeal Carcinoma.

Cancer informatics·2026

Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026

Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026

Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026

Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026

Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026

Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 8, 2026

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms

Published on: September 25, 2020

Mesh-based metasurfaces with high optical transparency.

Minghui Deng, Tiansheng Cui, Bozhen Hou

|December 19, 2025

Summary

This summary is machine-generated.

This study introduces a novel metasurface technology using fine-line meshes for high optical transparency and efficient electromagnetic absorption. The developed transparent microwave absorber demonstrates polarization insensitivity and wide-angle characteristics.

More Related Videos

Demonstration of Equal-Intensity Beam Generation by Dielectric Metasurfaces

Demonstration of Equal-Intensity Beam Generation by Dielectric Metasurfaces

Published on: June 7, 2019

Scalable Solution-processed Fabrication Strategy for High-performance, Flexible, Transparent Electrodes with Embedded Metal Mesh

Scalable Solution-processed Fabrication Strategy for High-performance, Flexible, Transparent Electrodes with Embedded Metal Mesh

Published on: June 23, 2017

Related Experiment Videos

Last Updated: Jan 8, 2026

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms

Published on: September 25, 2020

Demonstration of Equal-Intensity Beam Generation by Dielectric Metasurfaces

Demonstration of Equal-Intensity Beam Generation by Dielectric Metasurfaces

Published on: June 7, 2019

Scalable Solution-processed Fabrication Strategy for High-performance, Flexible, Transparent Electrodes with Embedded Metal Mesh

Scalable Solution-processed Fabrication Strategy for High-performance, Flexible, Transparent Electrodes with Embedded Metal Mesh

Published on: June 23, 2017

Area of Science:

Metamaterials and Nanophotonics
Electromagnetic Wave Manipulation

Background:

Traditional resonant patterns in metasurfaces often compromise optical transparency.
Achieving efficient electromagnetic absorption alongside high optical transmittance is a significant challenge.

Purpose of the Study:

To propose and demonstrate a novel metasurface technology for optically transparent electromagnetic absorbers.
To design a microwave absorber with polarization insensitivity and wide-angle incidence using fine-line mesh metasurfaces.

Main Methods:

A metasurface design utilizing a fine-line-based mesh resonant structure was developed.
An optically transparent microwave absorber was fabricated using nano-imprinting technology, comprising stacked mesh metasurface films.
The absorption rate was tuned by adjusting the stacking gap (transparent layer thickness).

Main Results:

The fabricated metasurface films achieved high average optical transmittances of 91.53% and 92.67%.
The developed absorber exhibited polarization insensitivity and wide-angle incidence characteristics.
Tunable electromagnetic absorption rates ranging from 94.15% to 18.4% were achieved by adjusting the transparent layer thickness.

Conclusions:

The proposed fine-line mesh metasurface technology enables simultaneous high optical transparency and efficient electromagnetic absorption.
Embedding these metasurface films into transparent materials offers a viable solution for applications requiring both functionalities, such as transparent windows.