Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

2.7K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
2.7K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.4K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.4K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

1.2K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.2K
Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

4.3K
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
4.3K
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

1.8K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.8K
IR Spectrum Peak Broadening: Hydrogen Bonding01:23

IR Spectrum Peak Broadening: Hydrogen Bonding

1.7K
The vibrational frequency of a bond is directly proportional to its bond strength. As a result, stronger bonds vibrate at higher frequencies, while weaker bonds vibrate at lower frequencies. The stretching vibration of the strong O–H bond in alcohols and phenols (very dilute solution or gas phase) appears as a sharp peak at 3600–3650 cm−1.
However, the extent of hydrogen bonding influences the observed stretching frequency and band broadening. Intermolecular or intramolecular...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Apoptosis-inducing effect and structural basis of Polygonatum cyrtonema lectin and chemical modification properties on its mannose-binding sites.

BMB reports·2008
Same author

The catalytic intermediate stabilized by a "down" active site loop for diaminopimelate decarboxylase from Helicobacter pylori. Enzymatic characterization with crystal structure analysis.

The Journal of biological chemistry·2008
Same author

Monitoring prostate thermal therapy with diffusion-weighted MRI.

Magnetic resonance in medicine·2008
Same author

Removal of ammonia nitrogen in wastewater by microwave radiation.

Journal of hazardous materials·2008
Same author

[Three-dimensional anatomical position of rotatory center in cervical rotatory and local manipulation].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2008
Same author

Dysregulation of CREB binding protein triggers thrombin-induced proliferation of vascular smooth muscle cells.

Molecular and cellular biochemistry·2008

Related Experiment Video

Updated: Jan 8, 2026

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
05:45

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

3.0K

Structural and dynamical phase jumps in molecular high harmonic spectroscopy.

Zheng Shu, Jing Chen

    Optics Express
    |December 19, 2025
    PubMed
    Summary

    High harmonic spectroscopy reveals molecular insights using spectral phase analysis. This study clarifies phase jumps from structural and dynamic interferences, advancing ultrafast molecular dynamics research.

    Area of Science:

    • Quantum physics
    • Molecular spectroscopy
    • Attosecond science

    Background:

    • High harmonic spectroscopy (HHS) offers attosecond-ångström resolution for molecular structure and dynamics.
    • Harmonic spectra contain information on molecular structure and dynamics via two-center and multichannel dynamic interference.
    • Spectral phase is crucial for wavefunction reconstruction and understanding ultrafast molecular dynamics.

    Purpose of the Study:

    • To comprehensively analyze phase jumps associated with structural and dynamic interferences in high harmonic generation.
    • To elucidate the physical origins of spectral phase variations in strong-field interactions.
    • To highlight the utility of spectral phase information for probing ultrafast molecular dynamics.

    Main Methods:

    • Utilizing the strong-field approximation (SFA) framework.

    More Related Videos

    Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
    10:35

    Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

    Published on: May 29, 2018

    9.1K
    Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
    14:55

    Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

    Published on: September 17, 2017

    15.9K

    Related Experiment Videos

    Last Updated: Jan 8, 2026

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
    05:45

    Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

    Published on: March 31, 2022

    3.0K
    Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
    10:35

    Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

    Published on: May 29, 2018

    9.1K
    Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
    14:55

    Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

    Published on: September 17, 2017

    15.9K
  • Conducting a detailed analysis of spectral phase jumps.
  • Investigating interference effects in high harmonic emissions.

    • Main Results:

    • Identified distinct phase jump behaviors linked to structural two-center and multichannel dynamic interference.
    • Revealed the physical origins behind observed spectral phase variations.
    • Demonstrated that spectral phase provides critical insights into interference mechanisms.

    Conclusions:

    • Spectral phase analysis is a powerful tool for understanding molecular structure and dynamics.
    • The study clarifies the relationship between interference phenomena and spectral phase.
    • Findings underscore the potential of spectral phase in ultrafast molecular dynamics research.