Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COX-2 inhibition improves immune system homeostasis and decreases liver damage in septic rats.

The Journal of surgical research·2009
Same author

Mass spectral characterization of organophosphate-labeled, tyrosine-containing peptides: characteristic mass fragments and a new binding motif for organophosphates.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2009
Same author

3D-SURFER: software for high-throughput protein surface comparison and analysis.

Bioinformatics (Oxford, England)·2009
Same author

Total arch replacement with stented elephant trunk technique: a proposed treatment for complicated Stanford type B aortic dissection.

Journal of cardiac surgery·2009
Same author

Top-emitting white organic light-emitting devices with a one-dimensional metallic-dielectric photonic crystal anode.

Optics letters·2009
Same author

[Detection of tick and tick-borne pathogen in some ports of Inner Mongolia].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2009
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jan 8, 2026

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.5K

Single quantum dot standard for confocal detection efficiency.

Bin Li, Xiaopeng Chen, Guofa Qu

    Optics Express
    |December 19, 2025
    PubMed
    Summary
    This summary is machine-generated.

    We developed a fast, quantitative method using single quantum dots to measure confocal microscope detection efficiency. This technique precisely determines efficiency by analyzing photon arrival times, simplifying performance assessment.

    More Related Videos

    Compact Quantum Dots for Single-molecule Imaging
    17:14

    Compact Quantum Dots for Single-molecule Imaging

    Published on: October 9, 2012

    18.6K
    Video-rate Scanning Confocal Microscopy and Microendoscopy
    14:10

    Video-rate Scanning Confocal Microscopy and Microendoscopy

    Published on: October 20, 2011

    28.5K

    Related Experiment Videos

    Last Updated: Jan 8, 2026

    Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
    12:57

    Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

    Published on: October 13, 2017

    9.5K
    Compact Quantum Dots for Single-molecule Imaging
    17:14

    Compact Quantum Dots for Single-molecule Imaging

    Published on: October 9, 2012

    18.6K
    Video-rate Scanning Confocal Microscopy and Microendoscopy
    14:10

    Video-rate Scanning Confocal Microscopy and Microendoscopy

    Published on: October 20, 2011

    28.5K

    Area of Science:

    • Optical microscopy
    • Quantum optics
    • Nanotechnology

    Background:

    • Accurate measurement of confocal microscope detection efficiency is vital for performance assessment but technically challenging.
    • Existing methods may be time-consuming or require complex calibration procedures.
    • Quantitative fluorescence imaging and super-resolution microscopy rely on precise detection efficiency values.

    Purpose of the Study:

    • To present a rapid and quantitative method for measuring confocal microscope detection efficiency.
    • To establish a convenient benchmark for routine microscope optimization and inter-laboratory comparisons.
    • To facilitate advancements in quantitative fluorescence imaging.

    Main Methods:

    • Utilizing the photoluminescence of a single quantum dot as a light source.
    • Analyzing the cross-correlation of photon arrival times from single exciton and biexciton states.
    • Implementing a method that requires less than 30 seconds for measurement.

    Main Results:

    • Precise determination of confocal microscope detection efficiency.
    • Method demonstrates high tolerance to variations in laser power.
    • No calibration procedures are necessary for this technique.
    • Measurements are completed in under 30 seconds.

    Conclusions:

    • The single quantum dot method offers a rapid, quantitative, and calibration-free approach to measure detection efficiency.
    • This technique serves as a valuable tool for routine microscope optimization and reliable comparisons.
    • The method lays a foundation for improved quantitative fluorescence imaging and super-resolution microscopy.