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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

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Published on: July 5, 2016

Spatial-shifting cepstrum: holography without a known reference beam.

Ricardo Rubio-Oliver, José Ángel Picazo-Bueno, Javier García

|December 19, 2025

Summary

This summary is machine-generated.

Spatial-shifting cepstrum (SSC) is a new digital holographic microscopy method. It allows conventional microscopes to perform DHM without a reference beam, offering unrestricted sample imaging.

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Area of Science:

Microscopy
Optical Physics
Digital Imaging

Background:

Digital holographic microscopy (DHM) typically requires specific conditions for interferometric beams and a predefined reference beam.
These constraints limit the application of DHM to various sample types and imaging scenarios.

Purpose of the Study:

To introduce a novel digital holographic microscopy (DHM) approach, the spatial-shifting cepstrum (SSC).
To overcome the limitations of conventional DHM by removing constraints on interferometric beams and the need for a reference beam.
To enable DHM with conventional microscopes using a minimal interferometric module.

Main Methods:

Development of the spatial-shifting cepstrum (SSC) technique.
Simulations to model and analyze the SSC method.
Experimental validation using optical setups and diverse samples.

Main Results:

SSC successfully removes constraints on interferometric beams and the requirement for a predefined reference beam.
Demonstrated robustness of SSC to spatial shift variations and sample absorptiveness.
Maintained quantitative phase accuracy in DHM measurements.
Conventional microscopes can perform DHM with minimal modifications.

Conclusions:

SSC is a flexible and universal solution for digital holographic microscopy.
The method allows for unrestricted sample properties and field of view in DHM.
Provides practical guidelines for implementation, algorithmic details, and integration strategies.