Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

2.7K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
2.7K
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

4.6K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
4.6K
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

4.4K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
4.4K
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

1.9K
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
1.9K
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

6.5K
The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
6.5K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

3.3K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photodynamics of amino acids under UV excitation: Extraterrestrial amino acids.

The Journal of chemical physics·2026
Same author

Muscle strength characteristics and the predictive value of handgrip strength in young obese males: A multi‑joint isokinetic and LASSO regression analysis.

PloS one·2026
Same author

Better adherence to the life's essential 8 can reduce the risk of gallstone disease: mediated by inflammation and oxidative stress.

Frontiers in public health·2026
Same author

Comparative transcriptome analysis reveals key genes and regulatory networks regulating flowering in rice varieties with different photoperiod sensitivity.

BMC plant biology·2026
Same author

Ultraviolet-driven self-repair in chimeric d(GAUU) outcompetes damage formation.

Chemical communications (Cambridge, England)·2026
Same author

Functional Nitrile Ether Additives for High-Energy-Density Lithium Metal Batteries: Multiscale Mechanism Study on Cyanide Substitution Regulation.

The journal of physical chemistry. B·2026

Related Experiment Video

Updated: Jan 8, 2026

Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems
06:54

Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems

Published on: June 23, 2023

1.3K

Quantitative model for infrared nano-spectroscopy in liquid.

Tarik Cigeroglu, Jia Zeng, Phillip Tran

    Optics Express
    |December 19, 2025
    PubMed
    Summary

    A new numerical model enhances nanoscale spectroscopy at liquid-solid interfaces. This method accurately interprets scattering-scanning near-field optical microscopy (s-SNOM) data, improving vibrational spectroscopy of thin films.

    More Related Videos

    Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere
    08:52

    Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere

    Published on: April 30, 2018

    8.6K
    Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
    10:42

    Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

    Published on: March 22, 2019

    6.6K

    Related Experiment Videos

    Last Updated: Jan 8, 2026

    Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems
    06:54

    Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems

    Published on: June 23, 2023

    1.3K
    Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere
    08:52

    Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere

    Published on: April 30, 2018

    8.6K
    Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
    10:42

    Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

    Published on: March 22, 2019

    6.6K

    Area of Science:

    • Physics
    • Chemistry
    • Materials Science

    Background:

    • Nanoscale spectroscopy at liquid-solid interfaces is advancing.
    • Current theoretical models for scattering-scanning near-field optical microscopy (s-SNOM) lack detailed probe geometry and use approximations.
    • Total internal reflection (TIR) geometry illumination is key to this technique.

    Purpose of the Study:

    • To develop a robust numerical model for TIR s-SNOM.
    • To overcome limitations of quasistatic approximations in existing models.
    • To enable quantitative interpretation of s-SNOM spectroscopy at interfaces.

    Main Methods:

    • Utilized a Maxwell's equation solver for optical field calculations.
    • Implemented a near-to-far-field transformation for signal analysis.
    • Applied the model to vibrational spectroscopy of thin-film proteins.

    Main Results:

    • The numerical model accurately predicts directional signals, breaking quasistatic approximations.
    • Strong agreement was found between calculated and experimental approach curves.
    • Quantified thickness-dependent signals and spectral shifts in protein films.

    Conclusions:

    • The developed far-field reconstruction approach provides quantitative interpretation for TIR s-SNOM.
    • This numerical modeling is expected to become a reliable tool for s-SNOM nanospectroscopy.
    • The method is applicable to diverse optical geometries and sample types.