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Published on: January 5, 2017
Enzymatically Hydrolyzed Wheat Bran-Derived Zinc Phytate Ameliorates Ulcerative Colitis via Synergistic Regulation of
Liansheng Zhang1, Mingfang Zhan2, Yan Zhao1
1Key Laboratory of Ministry of Education for Medicinal Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
Abstract:
This study evaluates the therapeutic potential of enzymatically hydrolyzed wheat bran-derived zinc phytate (EEPZ) in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mouse model, with chemically synthesized zinc phytate (ZnPA) and whole wheat bran (WHB) as controls. EEPZ modulated gut microbiota by increasing Lactobacillus vaginalis (0.15% to 18.6%) and reducing Desulfovibrio (5.19% to 1.29%). Metabolically, Lactobacillus vaginalis converted EEPZ or standard zinc phytate into bioavailable inositol phosphates (e.g., IP3, IP4, IP5) and ionic zinc in vitro and in vivo. Concomitantly, EEPZ altered short-chain fatty acid and bile acid profiles (Chenodeoxycholic acid increased by 30% and lithocholic acid increased by 80%). Mechanistically, EEPZ strengthened intestinal barrier integrity via HDAC3 and tight junction proteins (ZO-1, occludin) upregulation, while concurrently suppressing the PI3K/AKT/NF-κB signaling pathway. Collectively, EEPZ emerges as a novel, natural UC therapeutic candidate.
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