Association between NAD (P) H: Quinone Oxidoreductase 1 C609T Gene Polymorphism and Risk Factors in Cases of Cervical...

HPV infection is causative agent for cervical cancer, majority of HPV infections are low-grade, self-limited, and revert spontaneously in some HPV infection fails to clear this hints towards presence of certain genetic factors which predispose them to increase risk of pre-cancer and cancer. We aimed to investigate the factors which have bearing on rising cancer cases in India and to elucidate the genetic susceptibility distribution of NAD (P) H: quinone oxidoreductase 1 C609T gene polymorphism.

Study recruited 142 women with biopsy-proven cervical cancer (the case group) and 142 women with normal cytology (the control group). DNA extracted from blood via QIAamp DNA mini kit. The DNA of each participant was amplified and the genotype and allelic frequency were compared.

In our study, maximum women in the case group had stage II (69.7%) squamous cell carcinoma (97.2%). 75.4% of the cases had moderately differentiated carcinoma and had no treatment before (43.7%). Among cases, the prevalence of genotype CC, CT and TT was 42.3%, 19.7% and 38.0%, respectively compared to 54.9%, 13.4% and 31.7%, in controls. The odds of having CC (odds ratio [OR] =0.64; 95% confidence interval [CI] =0.38-1.08), CC or CT in contrast to TT (OR = 0.756; 95% CI = 0.46-1.23), CC in contrast to TT/CT (OR = 0.694; 95% CI = 0.44-1.10) was lesser in cases compared to the controls, while Odds of allele T as compared to C were significantly elevated in cases as compared to that in the controls (OR = 1.48; 95% CI = 1.06-2.06). The proportion of TT genotype was elevated in advanced stages as compared to early stages.

We found the odds of allele T as compared to C were markedly elevated in cases as compared to controls.