Related Experiment Video

Updated: Jan 8, 2026

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

32.1K

Expression profiling reveals coordinated dysregulation of autophagy-associated proteins in marginal zone lymphoma and

Jingshu Ruan1,2, Xin Zhou2, Ai Li1

  • 1Department of Hematology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.

Oncology Letters
|December 19, 2025
PubMed

Insights

Autophagy, a cellular process, is dysregulated in marginal zone lymphoma (MZL). Key protein changes in MZL suggest autophagy modulation as a potential therapeutic strategy for this lymphoma.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Medicine

Background:

  • Autophagy dysregulation is a key area in oncology research.
  • Limited studies exist on autophagy's role in marginal zone lymphoma (MZL) pathogenesis.
  • Targeting autophagy presents a potential therapeutic avenue in cancer treatment.

Purpose of the Study:

  • To characterize autophagy-related protein expression in MZL.
  • To investigate the role of autophagy in MZL pathogenesis.
  • To provide a rationale for therapeutic targeting of autophagy in MZL.

Main Methods:

  • Immunohistochemical analysis of Beclin-1, LC3, SQSTM1/p62, and Bcl-2.
  • Comparison of protein expression in MZL tissues versus reactive lymphoid hyperplasia (RLH) controls.
  • Analysis of formalin-fixed paraffin-embedded tissue samples from 16 MZL patients and 16 RLH controls.

Main Results:

  • MZL tissues showed significantly decreased expression of Beclin-1 and LC3 (~35-40%).
  • MZL tissues exhibited significantly increased expression of SQSTM1/p62 and Bcl-2 (~30-45%).
  • These changes indicate significant autophagy pathway dysregulation in MZL compared to RLH (P<0.05).

Conclusions:

  • MZL displays a distinct profile of autophagy dysfunction.
  • Aberrant expression of Beclin-1, LC3, SQSTM1/p62, and Bcl-2 may serve as disease biomarkers.
  • Modulating autophagy pathways holds therapeutic potential for marginal zone lymphoma.
Keywords:
LC3SQSTM1/p62autophagybeclin-1marginal zone lymphoma

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