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Genomic Evidence for Elevational Segregation and Adaptive Introgression in Prunellidae Radiation.

Xinlai Wu1,2, Wenqing Zang1,2, Per G P Ericson3

  • 1Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Molecular Ecology
|December 19, 2025
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Elevational segregation in mountain birds drives diversity. Adaptive introgression, particularly in low-elevation species, facilitated adaptation to higher altitudes and influenced evolutionary trajectories.

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Area of Science:

  • Evolutionary Biology
  • Genomics
  • Ornithology

Background:

  • Mountainous environments and climate change can drive speciation and adaptation in species.
  • The combined effects on biodiversity across large geographic areas are not well understood.

Purpose of the Study:

  • To investigate the genomic basis of diversification and adaptation in the avian family Prunellidae across Eurasian mountain ranges.
  • To explore the role of elevational gradients and glacial cycles in shaping genetic diversity and population dynamics.

Main Methods:

  • Comparative genomic analysis of Prunellidae species.
  • Analysis of genetic divergence (DXY) and genetic diversity across elevational gradients.
  • Demographic reconstructions to infer population responses to the Last Glacial Maximum.
  • Identification of genes under divergent and positive selection, and detection of interspecific introgression.

Main Results:

  • Low genetic divergence was observed among Prunellidae species.
  • High-elevation species exhibited greater genetic diversity and experienced population declines during the Last Glacial Maximum, with signatures of selection in genes related to stress response.
  • Low-elevation species showed population expansions, and introgression from low- to high-elevation lineages facilitated adaptation and range expansion.

Conclusions:

  • Elevational segregation is a key driver of montane avian diversity.
  • Adaptive introgression plays a significant role in the evolution of montane species, enabling adaptation to extreme environments and facilitating range shifts.