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Related Experiment Video

Updated: Jan 7, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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Photoswitchable Dual-Color Fluorescence With Large Stokes Shift From Dye-Encapsulated Metal-Organic Framework for

Chenyu Li1, He-Qi Zheng1, Weilu Xu2

  • 1State Key Laboratory of Silicon and Advanced Semiconductor Materials, Zhejiang Provincial Key Laboratory of Optoelectronic Functional Materials and Devices, ZJU-Hangzhou Global Scientific and Technological Innovation Center, School of Materials Science & Engineering, Zhejiang University, Hangzhou, China.

Advanced Materials (Deerfield Beach, Fla.)
|December 19, 2025
PubMed
Summary

Researchers developed a novel dual-color fluorescent material using metal-organic frameworks (MOFs) for advanced bioimaging. This material exhibits switchable emissions and a large Stokes shift, overcoming key challenges in fluorescence microscopy.

Keywords:
bioimagingcascade energy transferlarge Stokes shiftmetal‐organic frameworksphotoswitchable dual‐color fluorescence

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Area of Science:

  • Materials Science
  • Chemistry
  • Biotechnology

Background:

  • Photoswitchable dual-color fluorescent materials are crucial for cellular imaging and super-resolution microscopy.
  • Achieving a large Stokes shift in these materials remains a significant challenge.

Purpose of the Study:

  • To develop a novel strategy for photoswitchable dual-emission fluorescence with a large Stokes shift.
  • To create a dynamic luminescent material for advanced bioimaging applications.

Main Methods:

  • Utilized a cascade energy transfer (ET) process within metal-organic frameworks (MOFs).
  • Incorporated spiropyran as an ET intermediate, along with coumarin 153 (Cou153) and methylene blue (MB) dyes into a rho-ZMOF structure.
  • Investigated photoswitchable properties and emission characteristics under UV and visible light.

Main Results:

  • Achieved a dual-color dynamic luminescent material (rho-ZMOF⊃Cou153&SP&MB) exhibiting green (516 nm) and red (700 nm) fluorescence.
  • Demonstrated a maximum Stokes shift of 300 nm.
  • Showcased reversible switching of dual-color emissions via photoswitchable cascade energy transfer controlled by spiropyran's photochromism.

Conclusions:

  • The developed MOF-based material offers a promising solution for overcoming limitations in current fluorescent imaging techniques.
  • The material's photoswitchable dual-emission and large Stokes shift enable potential applications in high-resolution and depth-resolved bioimaging.