Identification of anti-inflammatory constituents of lindera glauca using cell extraction coupled with lC-QTOF MS/MS

Lindera glauca, an edible medicinal plant native to Northeastern Asia, has been reported to possess various pharmacological activities. This study employed cell extraction (CE) in conjunction with LC-QTOF-MS/MS to identify bioactive components that bind to target cells. The 80% MeOH extract of L. glauca exhibited significant nitric oxide (NO) production inhibitory activity in BV2 cells and was applied to the CE coupled with the LC-QTOF MS/MS method. New compound 3,5-dimethoxyphenyl-4-hydroxymethyl-β-ᴅ-glucopyranoside (2), and five known compounds (1, 3-6) were isolated from L. glauca. All compounds displayed anti-inflammatory activities, and compounds 2-3 inhibited NO production in BV2 cells. In molecular docking simulations, compound 2 showed high binding affinities to inflammatory marker proteins COX-2 and iNOS. In conclusion, the CE method combined with LC-QTOF MS/MS effectively facilitates the rapid identification of potential active compounds. And new compound 2 from L. glauca may be a new candidate against inflammatory diseases.