Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Driving Innovation: Transatlantic Attitudes to the <i>Bionics Bus</i> as a Vehicle for Health Transformation and STEM Engagement.

Healthcare technology letters·2026

Same author

Tumor extracellular vesicle RNA profiling predicts treatment response in pediatric diffuse midline glioma.

bioRxiv : the preprint server for biology·2026

Same author

Machine learning-assisted gait analysis for lameness detection in heterogeneous dog populations.

Veterinary journal (London, England : 1997)·2026

Same author

Scalable and Tunable 1D/2D Wrinkled MXene Topographies Regulate Osteoimmune Cell Interactions.

ACS applied bio materials·2026

Same author

Gut microbiota associate with disease severity and survival in idiopathic pulmonary fibrosis.

American journal of respiratory and critical care medicine·2026

Same author

In sepsis, circulating bacterial DNA correlates with disease severity and is filtered by the lungs.

American journal of respiratory and critical care medicine·2026

Same journal

Plasmonic nanocomposite helices for weather-adaptive LiDAR function.

Nature communications·2026

Same journal

Multidirectional strain-insensitive stretchable RF electronics.

Nature communications·2026

Same journal

In-scanner thoughts contribute to resting-state functional connectivity.

Nature communications·2026

Same journal

Metal-center electron affinity modulates multicolor electrochromism in 2D conjugated metal-organic frameworks.

Nature communications·2026

Same journal

Hyperbranched dielectric polymer networks exhibiting giant energy storage density at 250 °C.

Nature communications·2026

Same journal

3D nanoprinting of metals by spatiotemporally confined hot electrons via multiple-electron excitations in nanocrystals.

Nature communications·2026

See all related articles

Search research articles

Home
Midas: Rapid, Multiplexed Molecular Profiling For Integrated Host-pathogen Analysis.

Home
Midas: Rapid, Multiplexed Molecular Profiling For Integrated Host-pathogen Analysis.

Related Experiment Video

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

MIDAS: rapid, multiplexed molecular profiling for integrated host-pathogen analysis.

Yong Jun Lim¹, Mohammad Asadi Tokmedash², Matthew Allen³

¹Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.

Nature Communications

|December 19, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

A new platform, MIDAS, rapidly quantifies bacterial RNA and host inflammatory proteins simultaneously. This integrated molecular analysis offers a faster alternative to traditional methods for infectious disease research.

More Related Videos

A Multi-detection Assay for Malaria Transmitting Mosquitoes

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

Related Experiment Videos

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

A Multi-detection Assay for Malaria Transmitting Mosquitoes

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

Area of Science:

Biotechnology
Infectious Disease Diagnostics
Bioanalytical Chemistry

Background:

Integrated molecular analysis is crucial for complex infectious diseases, but current tools often analyze pathogens or host responses separately.
Sepsis research specifically needs rapid methods to quantify both microbial and host biomarkers for effective diagnosis and treatment.
Existing diagnostic workflows, such as microbial culture, are time-consuming, delaying critical clinical decisions.

Purpose of the Study:

To develop and present MIDAS (Multiplexed Intelligent Diffraction Analysis System), a novel platform for simultaneous host-pathogen molecular profiling.
To demonstrate the potential of MIDAS for rapid, integrated analysis in infectious conditions, particularly sepsis.
To evaluate the feasibility of MIDAS for potential point-of-care applications.

Main Methods:

Integration of shape-encoded hydrogel particles with lens-free diffraction imaging.
Application of deep learning algorithms for data analysis.
Simultaneous quantification of bacterial RNA and inflammatory proteins within a single assay system.

Main Results:

The MIDAS assay achieved multiplexed measurements in under 4 hours, significantly faster than traditional culture methods (20-40 hours).
High concordance was observed between MIDAS results and established methods (culture, qPCR, ELISA) in a porcine sepsis model.
The platform demonstrated successful simultaneous quantification of both bacterial RNA and host inflammatory proteins.

Conclusions:

MIDAS represents a proof-of-concept for a flexible, integrated host-pathogen profiling platform.
The system's speed and multiplexing capabilities offer a significant advancement over current diagnostic workflows.
Further clinical validation is needed, but MIDAS shows promise for accessible diagnostic tools in healthcare, agriculture, and food safety.