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Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Updated: Jan 7, 2026

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

Enhancing analytical sensitivity in upstream bioprocess using time-gated Raman spectroscopy.

Mahdi Mubin Shaikat¹, Venkata Gayatri Dhara², James K Drennen^1,3

¹Graduate School of Pharmaceutical Sciences, Duquesne University, Pittsburgh, PA, 15282, USA.

Bioprocess and Biosystems Engineering

|December 20, 2025

Summary

This summary is machine-generated.

Time-gated Raman spectroscopy (TGRS) effectively reduces fluorescence interference in mammalian cell cultures. This advancement improves signal-to-noise ratio and detection limits for critical analytes, enhancing upstream bioprocess monitoring.

Keywords:

Analytes Chinese hamster ovary cells Limit of detection Process analytical technology Signal to noise ratio Time-gated raman spectroscopy

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Published on: November 18, 2015

Area of Science:

Biotechnology
Analytical Chemistry
Process Analytical Technology (PAT)

Background:

Upstream bioprocessing relies on monitoring critical parameters like pH, dissolved oxygen, and analyte concentrations for metabolic state assessment.
Raman spectroscopy (RS) is a Process Analytical Technology (PAT) tool for inline monitoring, but suffers from fluorescence interference.
Fluorescence interference in RS reduces signal-to-noise ratio (SNR) and increases the limit of detection (LOD), hindering accurate analysis.

Purpose of the Study:

To evaluate Time-gated Raman Spectroscopy (TGRS) as a PAT tool for overcoming fluorescence interference in mammalian cell culture monitoring.
To assess the impact of TGRS on improving the SNR and LOD for key analytes in CHO cell cultures.
To demonstrate the enhanced detectability of analytes using TGRS for improved upstream bioprocess control.

Main Methods:

Application of Time-gated Raman Spectroscopy (TGRS) to mammalian cell culture samples.
Utilized pure component modeling and Net Analyte Signal (NAS) for data analysis.
Calculated Signal-to-Noise Ratio (SNR) and Limit of Detection (LOD) to quantify performance improvements.

Main Results:

TGRS significantly reduced fluorescence interference in Raman spectra.
The SNR and LOD for five key analytes in CHO cell culture samples were substantially improved.
Enhanced detectability of analytes was achieved, enabling more accurate process monitoring.

Conclusions:

Time-gated Raman Spectroscopy (TGRS) is a viable PAT tool for upstream bioprocessing.
TGRS overcomes the limitations of conventional RS by mitigating fluorescence interference.
This technology facilitates accurate monitoring and detection of analytes in complex bioprocess systems, improving process control.