Preparation, Characteristics, Antioxidant and Anti-Inflammatory Activity of SeNPs and ZnO-enriched...

Trace elements are crucial for animal development, but current additives like sodium selenite (Na₂SeO₃) and zinc sulfate (ZnSO₄) pose toxicity and low bioavailability risks. Additionally, finding alternatives to antibiotics is a pressing concern. This study used a safe and eco-friendly method to create SeNPs and ZnO-enriched Limosilactobacillus reuteri LR1 (L. reuteri LR1-SeNPs-ZnO). And its characteristics, antibacterial and antioxidant activities were studied. These results showed that Na₂SeO₃ and ZnSO₄ were transformed into SeNPs and ZnO by L. reuteri LR1, respectively. And it had significant bacteriostatic and antioxidant activities. Moreover, dietary supplementation of L. reuteri LR1-SeNPs-ZnO significantly increased the antioxidant and anti-inflammatory activities, and increased the expression level of tight junction protein in jejunum, and significantly improved intestinal microbiota dysbiosis induced by LPS. These results indicated that SeNPs and ZnO-enriched L. reuteri LR1 can replace antibiotics in maintaining intestinal health, with demonstrated benefits in enhancing antioxidant capacity and regulating inflammatory responses.