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Balancing accuracy and efficiency in peripheral blood stem cell collection: A protocol comparison study.

Nidhi Sharma1, Khushboo Likhar1, Sachin Sharma1

  • 1Immunohematology and Blood Transfusion, MGM Medical College, Indore, Madhya Pradesh, India.

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis
|December 20, 2025
PubMed
Summary

Pre-peripheral blood CD34+ cell count (PBCC) based protocols offer a more reliable method for predicting successful stem cell collection compared to intra-procedure CD34+ cell count (IPC) based methods. This approach balances accuracy and error control, reducing donor burden.

Keywords:
Collection efficiencyIntraprocedure countPeripheral Blood Stem Cell collectionPre-procedure CD34 + cell count

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Area of Science:

  • Hematology
  • Transplantation Medicine
  • Clinical Research

Background:

  • Peripheral blood stem cell (PBSC) collection efficiency is influenced by various factors.
  • Identifying these factors is crucial for optimizing stem cell collection success.
  • Current protocols rely on intra-procedure measurements, which may have limitations.

Purpose of the Study:

  • To identify factors influencing successful PBSC collection.
  • To compare the accuracy of pre-peripheral blood CD34+ cell count (PBCC) based predictive protocols against intra-procedure CD34+ cell count (IPC) based protocols.
  • To evaluate predictive algorithms for stem cell dose prediction.

Main Methods:

  • Retrospective analysis of 41 PBSC collections (31 allogeneic, 10 autologous).
  • Development of predictive algorithms using PBCC, CE2, intra-procedure CD34+ cell count (IPC), blood volume processed (nTBV), and recipient body weight.
  • Target doses set at 5x10^6 CD34+ cells/Kg (autologous) and 10x10^6 CD34+ cells/Kg (allogeneic).

Main Results:

  • PBCC and IPD showed strong associations with collection yield in autologous collections.
  • IPD was strongly associated with collection yield in allogeneic collections.
  • Pre-procedure FD (pFD) was the most accurate protocol for autologous collections; however, pFD and prTBV showed moderate correlation with higher error in allogeneic collections. Fractional procedure values improved accuracy.

Conclusions:

  • Intra-procedure CD34+ cell count (IPC)-based algorithms demonstrated strong correlations but suffered from poor error metrics, limiting reliability.
  • Pre-peripheral blood CD34+ cell count (PBCC)-based predictions offer a superior balance of accuracy, error control, and operational consistency.
  • PBCC-based prediction is preferred for minimizing donor burden and ensuring optimal PBSC processing.