Related Experiment Video
Updated: Jan 8, 2026
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Relation Between Gut Microbiome and Inflammatory Interleukins in Psoriasis Patients Compared to Healthy Controls: An
L Schneller-Pavelescu1, C Mora-Martínez2, M J Sánchez-Pujol3
1Departamento de Dermatología, Hospital Vega Baja, Orihuela, Alicante, Spain.
Background:
The gut microbiota interacts with the immune system and plays an important role in many inflammatory diseases such as psoriasis, although the exact mechanisms in this disease are not yet well understood.
Objectives:
To characterize differences in the microbiota between patients with psoriasis and healthy controls, and to assess the relationship between these differences and the interleukins involved in psoriasis.
Methods:
A cross-sectional observational study was conducted in which sociodemographic data, blood samples, and stool samples were collected from patients with psoriasis and healthy controls attending our center between June 2019 and May 2020. Cytokines (interleukin (IL) 17, 22, 23, 31, 33, 36, interferon (IFN) γ, and transforming growth factor (TGF) β) were analyzed using ELISA, and microbiota was analyzed through 16S amplicon sequencing.
Results:
Thirty-six patients and 24 controls were included. Absolute abundance analysis found a higher abundance of the phylum Synergistota in the control group (p<0.05). Differential abundance analysis found higher abundance of the genus Subdoligranulum and Lactobacillus, and the species Bacteroides plebeius (p<0.05), and lower abundance of the species Senegalimassilia anaerobia and the genus Ruminococcus (p<0.05) in the psoriasis group. A relationship was observed between Subdoligranulum and TNFα, IL17, IL22, IL23, IL31, IL33, IL36, IFNγ, and TGFβ (p<0.05), as well as between Lactobacillus and IL17, IL23, IL36, TNFα, and TGFβ (p<0.05).
Conclusions:
Significant alterations in the gut microbiota of patients with psoriasis were detected and a relationship with inflammatory interleukins, suggesting their involvement in the disease. These findings could aid in the development of future probiotic treatments for psoriasis.
Insights
Psoriasis patients show altered gut bacteria, including differences in Subdoligranulum and Lactobacillus abundance, linked to inflammatory cytokines. These gut microbiota changes may offer new avenues for psoriasis treatment.
Area of Science:
- Immunodermatology
- Microbiome Research
- Gastroenterology
Background:
- The gut microbiota plays a crucial role in immune system regulation and inflammatory conditions.
- Psoriasis is an inflammatory disease where the gut microbiota's specific role and mechanisms remain unclear.
Purpose of the Study:
- To investigate and characterize the differences in gut microbiota composition between individuals with psoriasis and healthy controls.
- To explore the association between observed gut microbiota alterations and key interleukins implicated in psoriasis pathogenesis.
Main Methods:
- A cross-sectional observational study involving 36 psoriasis patients and 23 healthy controls.
- Analysis of gut microbiota via 16S amplicon sequencing and quantification of serum cytokines (IL-17, IL-22, IL-23, IL-31, IL-33, IL-36, IFN-γ, TGF-β) using ELISA.
Main Results:
- Significant differences in gut microbiota were found, with higher abundance of Synergistota in controls.
- Psoriasis patients exhibited increased levels of Subdoligranulum, Lactobacillus, and Bacteroides plebeius, and decreased levels of Senegalimassilia anaerobia and Ruminococcus.
- A notable correlation was observed between Subdoligranulum and Lactobacillus abundances and several inflammatory cytokines, including IL-17, IL-23, and TNF-α.
Conclusions:
- The gut microbiota is significantly altered in psoriasis patients, with specific bacterial taxa correlating with inflammatory cytokine profiles.
- These findings highlight the gut microbiota's involvement in psoriasis and suggest potential for novel probiotic-based therapeutic strategies.
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