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Published on: December 7, 2021
Isolating a novel quorum quenching strain based on carbon source adaptability for bioaugmentation under different
Jun Li1, Yanan Cui1, Fan Yang1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.
A novel Bacillus strain, YN06, effectively controls sludge bulking by degrading quorum quenching signals and outcompeting activated sludge for carbon sources. This bioaugmentation strategy enhances QQ activity and sludge treatment efficiency.
Area of Science:
- Environmental Microbiology
- Biotechnology
- Wastewater Treatment
Background:
- Bioaugmentation effectiveness is limited by environmental carbon sources.
- Sludge bulking is a persistent challenge in wastewater treatment.
- Quorum quenching (QQ) is a promising strategy for sludge bulking control.
Purpose of the Study:
- Isolate and characterize a novel QQ strain with strong carbon source utilization.
- Investigate the QQ mechanisms and carbon metabolism of the isolated strain.
- Evaluate the efficacy of the strain for sludge bulking control via bioaugmentation.
Main Methods:
- Isolation of QQ bacteria based on QQ activity and carbon source utilization.
- Degradation assays for N-acyl homoserine lactones (AHLs).
- Ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) and whole-genome sequencing.
- Molecular docking and 13C labeling experiments.
- Immobilization of bacteria and application in lab-scale reactors.
Main Results:
- Bacillus sp. YN06 efficiently degrades AHLs and utilizes glucose, sodium acetate, and alanine.
- YN06 possesses both lactonase and acylase pathways for AHL degradation, with specific enzymes identified.
- YN06 demonstrates superior carbon source competitiveness compared to activated sludge.
- Immobilized YN06 effectively controls sludge bulking by reducing extracellular polymeric substances.
Conclusions:
- A novel strategy for selecting QQ bacteria based on carbon source utilization was developed.
- Bacillus sp. YN06 is a promising candidate for bioaugmentation in sludge bulking control.
- Understanding carbon source competition is crucial for optimizing bioaugmentation strategies.
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