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Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

Published on: September 4, 2012

Single-cell transcriptomics reveals an abnormal immune microenvironment in plasma cell mastitis.

Qingtao Ni¹, Gaohua Han¹, Chi Pan²

¹Department of Oncology, The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou School of Clinical Medicine, Nanjing Medical University, Taizhou, China.

Annals of Medicine

|December 20, 2025

Summary

This study provides a detailed cellular and molecular analysis of plasma cell mastitis (PCM), revealing key immune cell alterations and gene expression changes. The findings offer a foundational resource for understanding PCM pathogenesis and developing new treatments.

Keywords:

Single-cell transcriptomics immune microenvironment plasma cell mastitis signalling pathway

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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Area of Science:

Immunology
Genomics
Cell Biology

Background:

Plasma cell mastitis (PCM) is a non-bacterial inflammatory breast disorder affecting women of reproductive age.
A comprehensive analysis of the cellular and molecular landscape of PCM is lacking.

Purpose of the Study:

To conduct a systematic, high-resolution analysis of the cellular and molecular characteristics of plasma cell mastitis.
To identify key cell types, gene expression patterns, and cellular interactions in PCM tissue.

Main Methods:

Utilized Comprehensive Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) on 10,185 cells from PCM tissue.
Performed differential gene expression analysis, functional enrichment, and cellular communication analysis.

Main Results:

Generated a detailed transcriptomic atlas of PCM, identifying thirteen distinct cell clusters and annotating eight cell types, including endothelial cells, common myeloid progenitor cells, and neutrophils.
Revealed significant alterations in immune cell composition and differential gene expression between normal and PCM samples.
Analyzed cellular communication networks to understand cell-to-cell interactions within the PCM microenvironment.

Conclusions:

This study presents a systematic and valuable perspective on the cellular and molecular mechanisms underlying plasma cell mastitis.
The generated data serves as a fundamental resource for future research into PCM pathogenesis, associated diseases, and potential therapeutic interventions.