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Updated: Jun 28, 2026

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
Published on: December 22, 2014
Unmasking the mimic: vertebral alveolar echinococcosis diagnosed by metagenomic next-generation sequencing
Tassilo Kruis1,2,3, Marion Wassermann4, Barbara Graf5
1Labor Berlin Charité Vivantes GmbH, Berlin, Germany. tassilo.kruis@laborberlin.com.
Abstract:
A Siberian woman in her forties presented to a public hospital in northeastern Germany with chronic back pain and a paravertebral mass, initially misdiagnosed as spinal tuberculosis. Repeated biopsies and metagenomic next-generation sequencing (mNGS) ultimately confirmed vertebral alveolar echinococcosis. Haplotype analysis revealed a novel Asian-cluster variant, supporting the presumed origin of infection.
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