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Related Concept Videos

Cluster Sampling Method01:20

Cluster Sampling Method

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Appropriate sampling methods ensure that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
To choose a cluster sample, divide the population into clusters (groups) and then randomly select some of the clusters. All the members from these clusters are in the cluster sample. For example, if you randomly sample four departments from your...
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Related Experiment Video

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The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
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Validation of a representative sampling protocol for studying grape berry microbiota at the plot scale.

Gabriela Pinto Miguel1, Olivier Rué2, Martine Pradal1

  • 1SPO, INRAE, Institut Agro, University of Montpellier, 2 Place Pierre Viala, 34060 Montpellier, France.

Journal of Microbiological Methods
|December 21, 2025
PubMed
Summary

Understanding grape microbiota is key for winemaking. This study compared two sampling methods for grape berry microbiota, finding that pooling samples impacts microbial diversity analysis.

Keywords:
DiversityGrape berriesMetabarcodingMicrobiotaSamplingYeast

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Area of Science:

  • Viticulture and Enology
  • Microbiology
  • Genetics

Background:

  • Grape microbiota significantly impacts viticulture, influencing vine health, grape development, fermentation, and wine sensory profiles.
  • Accurate monitoring of microbial communities is essential for optimizing vineyard management and ensuring wine authenticity.
  • Metagenetics offers a rapid, culture-independent method for identifying microorganisms in grape berry microbiota.

Purpose of the Study:

  • To evaluate the representativeness of different sampling methods for analyzing grape berry microbiota diversity at the plot level.
  • To compare the microbial community profiles obtained from pooled versus individually sampled grape berries.
  • To assess the impact of sampling strategy on understanding the diversity of the Artaban grape variety microbiota.

Main Methods:

  • Investigated the microbiota of the Artaban grape variety, known for its resistance to powdery mildew and tolerance to downy mildew.
  • Compared two distinct sampling methods: pooling microbiota from 10 vines versus analyzing each vine individually.
  • Utilized metagenetics for culture-independent identification of microbial communities at the genus or species level.

Main Results:

  • Pooling grape berry samples from multiple vines may alter the perceived microbial diversity compared to individual vine analysis.
  • Differences in microbial community composition were observed between the pooled and individual sampling approaches.
  • The study highlights the importance of sampling strategy in accurately characterizing vineyard microbiota.

Conclusions:

  • The choice of sampling method significantly influences the characterization of grape berry microbiota diversity.
  • Careful consideration of sampling techniques is necessary for reliable metagenetic studies in viticulture.
  • Optimized sampling protocols are crucial for accurate vineyard microbial monitoring and wine production insights.