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Global Transcriptomic Analysis Identifies Gene Expression Programs Regulated by α-Factor in Yeast.

Soojin Yeom1,2,3,4, Jung-Shin Lee1,2

  • 1Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of Korea.

Journal of Microbiology and Biotechnology
|December 21, 2025
PubMed
Summary
This summary is machine-generated.

This study reveals genome-wide gene expression changes in yeast cells responding to alpha-factor pheromone. It identifies hundreds of genes involved in mating, cell wall remodeling, and cell cycle regulation.

Keywords:
Saccharomyces cerevisiaecell cycle arrestchromatin remodelingpheromone signalingα-factor

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Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Cellular Signaling

Background:

  • Yeast mating pheromone alpha-factor induces a signaling cascade in haploid "a" cells.
  • Known physiological responses include G1 cell cycle arrest, polarized growth (shmoo formation), and cell fusion.
  • A comprehensive genome-wide transcriptional analysis was previously lacking.

Purpose of the Study:

  • To perform a genome-wide analysis of transcriptional changes in "a" mating type yeast cells treated with alpha-factor.
  • To identify differentially expressed genes (DEGs) in response to pheromone signaling.
  • To provide a resource for understanding yeast mating responses.

Main Methods:

  • RNA sequencing (RNA-seq) was employed to profile gene expression.
  • Yeast "a" mating type cells were treated with alpha-factor.
  • Gene ontology (GO) analysis was used to interpret functional enrichment of DEGs.

Main Results:

  • Identified 957 differentially expressed genes (DEGs): 448 upregulated and 509 downregulated.
  • Upregulated genes are enriched in pheromone signaling, cell wall biogenesis, and cell shape regulation.
  • Downregulated genes are associated with cell cycle progression, chromatin remodeling, and nucleosome organization.

Conclusions:

  • The study provides a valuable dataset of yeast pheromone-responsive genes.
  • Novel candidate genes involved in mating responses were identified.
  • Findings offer insights into the coordination of transcriptional regulation and chromatin dynamics during yeast pheromone signaling.