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QTL mapping for hundred-seed weight in soybean using high-density SLAF-seq technology
Chunlei Zhang1, Huilong Hong2, Rongqiang Yuan1
1Soybean Research Institute of Heilongjiang Academy of Agriculture Sciences, Harbin, 150086, China.
BMC Plant Biology
|December 21, 2025
Summary
Researchers mapped quantitative trait loci (QTLs) for soybean hundred-seed weight, identifying 11 significant QTLs and a novel major locus on chromosome 19. This work provides valuable molecular markers for improving soybean seed weight and yield through marker-assisted selection.
Area of Science:
- Plant Genetics
- Agricultural Science
- Soybean Breeding
Background:
- Hundred-seed weight is a crucial trait in soybean, impacting grain yield and market value.
- Understanding the genetic basis of seed weight is essential for developing improved soybean varieties.
Purpose of the Study:
- To dissect the genetic architecture of soybean hundred-seed weight variation.
- To identify quantitative trait loci (QTLs) and candidate genes associated with seed weight.
Main Methods:
- A recombinant inbred line (RIL) population of 325 lines was used for quantitative trait loci (QTL) mapping.
- High-density genotyping was performed using specific-locus amplified fragment sequencing (SLAF-seq).
- Multi-environment field trials were conducted, and candidate genes were analyzed using qRT-PCR.
Main Results:
- Eleven significant QTLs for hundred-seed weight were identified across eight chromosomes.
- A major QTL, qSW19-1, on chromosome 19 was detected with high significance, explaining 8.59% of phenotypic variance.
- Candidate gene analysis identified Glyma.19G195400 (cell wall invertase) as a promising candidate, with differential expression observed between parents.
Conclusions:
- The study expands the understanding of genetic factors controlling soybean seed weight.
- Identified QTLs and candidate genes offer potential for marker-assisted selection in soybean breeding programs.
- Functionally validated candidate genes provide targets for future research into seed weight regulation.
