An Opportunity for ISAC and ICCS to Lead on Universal Cytometry Standards

Flow cytometry, a complex and important methodology, is exacerbating the systemic reproducibility crisis in biological research. Our systematic review of 100 published manuscripts on PD-1 flow cytometry data found systemic deficiencies in the clarity and completeness of methodological documentation across top-tier journals. We urgently request that the International Society for Advancement of Cytometry (ISAC) and dedicated journal editors from the scientific publishing community lead a global initiative mandating a unique common standard for all submissions (MIFlowCyt). This adoption is critical for enforcing transparency, enabling rigorous peer review, and fundamentally strengthening the scientific integrity of published cytometry data.