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A near-infrared benzothiazole-based fluorescent probe for selective detection of CORM-3 with a large Stokes shift in

Liqiang Yan1, Junjie Yang1, Yanxin Zhang1

  • 1College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541006, China. liqiangyan@glut.edu.cn.

The Analyst
|December 22, 2025
PubMed

Insights

A new near-infrared fluorescent probe enables rapid and selective detection of Tricarbonyl chloride (glycine) ruthenium (CORM-3), a carbon monoxide (CO)-releasing agent. This tool aids in tracking CORM-3 in biological samples and developing new therapies.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Medical Chemistry

Background:

  • Carbon monoxide (CO) is a vital signaling molecule in mammals, crucial for cellular homeostasis and possessing therapeutic potential.
  • Dysregulation of CO metabolism is linked to various pathologies, highlighting the need for CO-releasing agents like Tricarbonyl chloride (glycine) ruthenium (CORM-3).
  • Effective detection methods for CORM-3 are essential for monitoring its levels and understanding its in vivo biological mechanisms.

Purpose of the Study:

  • To develop a novel near-infrared fluorescent probe for the selective and sensitive detection of CORM-3.
  • To assess the probe's performance in terms of response time, selectivity, sensitivity, and fluorescence properties.
  • To demonstrate the probe's utility in detecting CORM-3 in living cells and for on-site applications.

Main Methods:

  • Synthesis of a near-infrared fluorescent probe featuring a benzothiazole fluorophore and an allyl ether reactive site.
  • Evaluation of the probe's sensing performance, including response kinetics, selectivity against potential interferents, and fluorescence characteristics (turn-on signal at 660 nm).
  • Application of the probe for visual detection in living cells and fabrication into test strips for smartphone-based on-site analysis.

Main Results:

  • The developed probe enables rapid and selective detection of CORM-3 through a turn-on fluorescence mechanism at 660 nm.
  • The probe exhibits excellent sensitivity, a large Stokes shift, and avoids interference from heavy metal ions and nitroreductase.
  • Successful visual detection of CORM-3 in living cells was achieved, and the probe was adapted for convenient on-site detection using test strips and a smartphone application.

Conclusions:

  • The novel near-infrared fluorescent probe is a powerful tool for tracking CORM-3 in biological samples.
  • The probe offers advantages over existing methods by avoiding common limitations and enabling versatile detection formats.
  • This development facilitates the study of CORM-3's biological roles and supports its therapeutic applications.

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