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Diabetic Nephropathy01:28

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Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration occur due to afferent arteriolar...

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Prognostically Significant Glycan Profiles of Clear Cell Renal Cell Carcinoma Identified by Integrated

Yoshiko Kitazume1, Eri Arai1, Mao Fujimoto1

  • 1Department of Pathology, Keio University School of Medicine, Tokyo, Japan.

Pathology International
|December 22, 2025
PubMed
Summary

Clear cell renal cell carcinoma (ccRCC) shows immature N-type glycans. Glycan profiles, potentially epigenetically regulated, impact patient survival, offering clinical insights for ccRCC management.

Keywords:
Infinium assayclear cell renal cell carcinomaglycanglycogenelectin microarray

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Area of Science:

  • Oncology
  • Glycomics
  • Epigenetics

Background:

  • Clear cell renal cell carcinoma (ccRCC) is the most common subtype of kidney cancer.
  • Alterations in glycosylation are common in cancer, but their specific role in ccRCC pathogenesis and prognosis remains to be fully elucidated.
  • Understanding the clinicopathological significance of glycan profiles in ccRCC is crucial for developing novel diagnostic and prognostic biomarkers.

Purpose of the Study:

  • To investigate the clinicopathological significance of glycan profiles in clear cell renal cell carcinoma (ccRCC).
  • To explore the relationship between glycan profiles, epigenetic regulation, and patient outcomes in ccRCC.
  • To determine if comprehensive glycan profiling can provide clinically useful information for ccRCC patients.

Main Methods:

  • Glycomic and methylome analyses were performed on 50 paired nontumorous and tumorous renal tissue specimens from ccRCC patients.
  • Lectins recognizing high-mannose glycans, fucose, and sialic acid were used to assess glycan profiles.
  • Hierarchical clustering was applied to glycan signal intensities to stratify ccRCC samples.

Main Results:

  • Tumorous tissues exhibited higher high-mannose glycan signals and lower fucose/sialic acid signals compared to nontumorous tissues, indicating immature N-type glycans in ccRCC.
  • Hierarchical clustering identified two clusters (A and B) of ccRCC samples based on glycan profiles, with Cluster B associated with higher tumor-related death and lower overall survival (p=0.017 and p=0.019, respectively).
  • Specific glycan residues (asialo-type and N-acetyllactosamine) recognized by lectins DSA, ECA, and PHA-E significantly impacted recurrence-free and overall survival.

Conclusions:

  • The glycan profile of ccRCC is characterized by immature N-type glycans, potentially due to epigenetic regulation of glycosylating enzymes (glycogenes).
  • Distinct glycan profiles correlate with patient survival outcomes, suggesting prognostic value.
  • Comprehensive glycan profiling holds promise as a clinically valuable tool for assessing ccRCC patient prognosis and guiding treatment decisions.