Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LORA: a polymorphic multi-sample long read assembly pipeline.

NAR genomics and bioinformatics·2026
Same author

Supporting-like cells constitute an alternative steroidogenic lineage conserved in amniotes.

bioRxiv : the preprint server for biology·2026
Same author

[HIV-1 translational inventory reveals about a hundred of alternative ORFs and new antigens].

Medecine sciences : M/S·2026
Same author

Carbon Metabolic Versatility of Agrobacterium tumefaciens in Tomato Roots and Galls.

Environmental microbiology·2026
Same author

Bacterial chromatin remodeling associated with transcription-induced domains at pathogenicity Islands.

Nature communications·2026
Same author

Translon: a single term for translated regions.

Nature methods·2025

Related Experiment Video

Updated: Jan 8, 2026

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.7K

High-resolution HIV-1 m6A epitranscriptome reveals isoform-dependent methylation clusters and unique 2-LTR transcript

Delphine Naquin1, Sandra Blanchet1, Erwin van Dijk1

  • 1Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette 91198, France.

NAR Genomics and Bioinformatics
|December 22, 2025
PubMed
Summary
This summary is machine-generated.

Researchers precisely mapped N6-methyladenosine (m6A) sites on HIV-1 RNA using Nanopore sequencing. This revealed 18 m6A sites, including a novel RNA species with highly methylated sites potentially crucial for HIV-1 infection.

More Related Videos

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.2K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.3K

Related Experiment Videos

Last Updated: Jan 8, 2026

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.7K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.2K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.3K

Area of Science:

  • Virology
  • Molecular Biology
  • Epigenetics

Background:

  • N6-methyladenosine (m6A) is a key RNA modification in HIV-1.
  • Previous detection methods lacked precision, leaving the exact number and location of m6A sites unclear.

Purpose of the Study:

  • To precisely identify and map m6A modification sites on HIV-1 RNA.
  • To investigate differential methylation patterns across splicing isoforms.
  • To characterize a novel RNA species derived from circular DNA and its m6A sites.

Main Methods:

  • Utilized Nanopore sequencing with direct m6A base-calling.
  • Performed single-molecule analysis of RNA transcripts.
  • Analyzed RNA species transcribed from circular viral DNA.

Main Results:

  • Identified 18 m6A sites on HIV-1 RNA: 14 in the 3' region and 4 centrally.
  • Observed differential methylation across splicing isoforms and clustered methylation profiles.
  • Discovered a ~732 nt RNA species with six m6A sites, five highly methylated and one unique, suggesting a specific role in infection.

Conclusions:

  • Provides a high-resolution map of the HIV-1 m6A transcriptome.
  • Highlights a novel RNA species and unique m6A sites with potential significance in the HIV-1 life cycle.
  • Establishes a foundation for future research into the functional roles of m6A modifications in HIV-1.