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Related Experiment Video

Updated: Jan 8, 2026

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Published on: June 7, 2019

Histone-related gene WDR77 promotes tumor progression through cell cycle regulation in skin cutaneous melanoma.

Haoxue Zhang^1,2,3, Ke Tang^1,2,3, Yuyao Liu^1,2,3

¹Department of Dermatovenerology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.

Frontiers in Immunology

|December 22, 2025

Summary

WDR77 is a key gene in skin cutaneous melanoma (SKCM) that promotes tumor growth and is linked to poor prognosis. Targeting WDR77 may offer new therapeutic strategies for melanoma patients.

Keywords:

CDC20 WDR77 cell cycle histone melanoma prognosis biomarker therapeutic target

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Last Updated: Jan 8, 2026

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

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Published on: June 7, 2019

The Soft Agar Colony Formation Assay

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Published on: October 27, 2014

A 3D Organotypic Melanoma Spheroid Skin Model

A 3D Organotypic Melanoma Spheroid Skin Model

Published on: May 18, 2018

Area of Science:

Oncology
Molecular Biology
Epigenetics

Background:

Skin cutaneous melanoma (SKCM) is an aggressive cancer with poor prognosis.
Epigenetic alterations, especially histone modifications, drive melanoma progression.
There is a clinical need for effective histone-based prognostic biomarkers in SKCM.

Purpose of the Study:

To identify novel histone-related prognostic biomarkers for SKCM.
To investigate the functional role of candidate genes in melanoma.
To explore the therapeutic potential of identified biomarkers.

Main Methods:

Analysis of TCGA-SKCM and GEO transcriptomic data.
Development and validation of 101 prognostic models using machine learning.
Identification of WDR77 as a hub gene via random forest analysis.
In vitro and in silico functional validation of WDR77.

Main Results:

WDR77, a histone methylation factor, was significantly upregulated in SKCM and associated with poor survival.
High WDR77 and CDC20 expression correlated with the worst patient outcomes.
WDR77 promotes melanoma cell proliferation, migration, and cell cycle progression, upregulating CDC20.

Conclusions:

WDR77 is a crucial prognostic biomarker and functional regulator in melanoma.
WDR77 plays a significant role in cell-cycle dysregulation in SKCM.
WDR77 represents a potential therapeutic target for melanoma treatment.