The StTNLC7G2T1 enhances resistance to biotic stress in Arabidopsis via pathogen triggered immunity
Namo Dubey1,2, L Mathan1, Sidharth Patidar1
1Present Address: CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176061 India.
Abstract:
The nucleotide binding site-leucine rich repeat (NBS-LRR) gene family is widely recognized for playing prominent role in plant defence against diverse pathogens. One such member, StTNLC7G2T1 (PGSC0003DMC400041857) from the cultivated potato (Solanum tuberosum) variety Kufri Jyoti was functionally characterized to investigate its role in plant immunity. Transgenic Arabidopsis plants were raised expressing StTNLC7G2T1 (OEStTNLC7G2T1) exhibiting significantly enhanced resistance to fungal pathogen Alternaria brassicicola and bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Compared to wild-type (Col-0) plants, these transgenic lines showed induced callose deposition at infection sites, suggesting a strengthened cell wall-mediated defence response. The OEStTNLC7G2T1 plants maintained photosynthetic efficiency under pathogen attack, potentially contributing to improved overall plant health and resilience. The transcript expression patterns revealed significant modulation of genes associated with Salicylic acid (SA) and Jasmonic acid (JA) mediated defence responses (PR1, PR2, NPR1, VSP2, PDF1.2 and MYC2), indicating that StTNLC7G2T1 may enhance plant immunity by influencing hormone signaling pathways. The observed increase in callose accumulation and the expression profile of select genes revealed that transgenic StTNLC7G2T1 confers pathogen-triggered immunity in Arabidopsis via enhanced basal defense.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s12298-025-01681-4.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...


