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Transglutaminase 7 Silencing Inhibits Proliferation and Modulates Inflammatory and Apoptotic Markers in Testicular

Rawabi S Altuwayjiri1, Ibtesam S Almami1

  • 1Department of Biology, College of Science, Qassim University, Buraydah, 52571, Al-Qassim, Saudi Arabia.

Oncology Research
|December 22, 2025
PubMed
Abstract

Insights

Transglutaminase 7 (TG7) is overexpressed in testicular germ cell tumors (TGCTs) and promotes cancer cell survival. Silencing TG7 reduces tumor cell viability and modulates inflammation and apoptosis, identifying TG7 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Testicular germ cell tumors (TGCTs) are the most common cancer in young men (20-40 years).
  • The role of Transglutaminase 7 (TG7) in TGCT pathogenesis is largely unknown.
  • TG7 is an enzyme encoded by the TGM7 gene with poorly characterized functions.

Purpose of the Study:

  • To evaluate TG7 protein expression in clinical TGCT specimens.
  • To investigate the functional role of TG7 in TGCT using the NT2/D1 cell line.
  • To determine TG7's impact on cell viability, inflammation, and apoptosis pathways.

Main Methods:

  • Immunohistochemistry (IHC) and immunofluorescence (IF) for TG7 protein evaluation.
  • Dicer-substrate small interfering RNAs (DsiRNAs) for TG7 gene silencing in NT2/D1 cells.
  • qRT-PCR for gene expression analysis, MTT assay for cell viability, and IF for protein suppression validation.

Main Results:

  • TG7 expression was significantly increased (approx. 4.5-fold) in TGCT tissues compared to normal testis.
  • TG7 knockdown in NT2/D1 cells dose-dependently reduced cell viability by up to 48%.
  • TG7 silencing upregulated IL6, TNFα, and CASP3 mRNA levels, indicating modulation of inflammation and apoptosis via p53-independent pathways.

Conclusions:

  • TG7 is significantly overexpressed in TGCT and supports tumor cell viability in vitro.
  • TG7 represents a novel biomarker and potential therapeutic target for testicular cancer.
  • Further research into TG7-targeted interventions for testicular cancer is warranted.
Keywords:
Human malignantgene expressionknockdownproliferationtesticular germ cell linetransglutaminase 7

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