A Tutorial Review on Surface Plasmon Resonance Biosensors: Applications in Biomedicine

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensor Technology

Background:

• Surface Plasmon Resonance (SPR) is a key technology for analyzing macromolecular interactions, offering high specificity and affinity.
• SPR biosensors are crucial for real-time, label-free detection of biomolecules.
• Recent innovations focus on improving SPR for early disease diagnosis and monitoring.

Purpose of the Study:

• To provide an overview of recent advances and applications of SPR biosensors in biomedicine.
• To highlight the potential of SPR for detecting low-abundance compounds relevant to disease prevention and early diagnosis.
• To discuss technical innovations and their impact on SPR device performance.

Main Methods:

• Review of recent technical innovations in SPR devices, including miniaturization and cost reduction.
• Exploration of advanced SPR techniques like Localized SPR (LSPR), SPR imaging (SPRi), and SPR microscopy (SPRM).
• Analysis of SPR biosensor applications in detecting biomarkers, viruses, toxins, drugs, and hormones.

Main Results:

• SPR biosensors enable real-time, label-free detection of various biomolecules.
• Advances have led to miniaturized, portable, and cost-effective SPR devices.
• New SPR techniques (LSPR, SPRi, SPRM) aim to enhance sensitivity and detection performance.

Conclusions:

• SPR biosensors are highly effective for evaluating macromolecular interactions and detecting clinically relevant compounds.
• Technological advancements are expanding the utility of SPR in point-of-care diagnostics and disease monitoring.
• SPR technology shows significant promise for the early detection of diseases through sensitive biomarker analysis.