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Anti-interferon-γ Autoantibodies and Indeterminate QuantiFERONⓇ-TB Gold Plus Assays

Serena Lee1, Annalie J Harris1, Christa S Zerbe1

  • 1Laboratory of Clinical Immunology and Microbiology, NIAID, NIH, Bethesda, Maryland, USA.

Open Forum Infectious Diseases
|December 22, 2025
PubMed

Among 852 samples sent for QuantiFERON®-TB Gold Plus Assay (QFN) analysis, between 6% and 11% had IFNγ binding activity, but only 1.2% of indeterminate samples had IFNγ blocking activity. No TB negative samples had blocking activity to IFNγ. Indeterminate QFN results may indicate the presence of blocking anti-IFNγ autoantibodies.

Keywords:
QuantiFERON-TB Gold Plusanti–cytokine autoantibodiesindeterminate IGRA

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