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Updated: Jan 8, 2026
Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Preparation and functional validation of rabbit anti-canine CD3ε monoclonal antibody
Huixin Li1,2, Mengjuan Chen1,2, Rongmiao Yue1,2
1College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
Introduction:
CD3, a surface antigen critical for T cell activation and signal transduction, serves as a key diagnostic marker for T cell-associated malignancies and a therapeutic target in immunotherapy. With canine models gaining prominence in translational immunology and oncology, reliable tools to study T cell-mediated immunity are essential.
Methods And Results:
In this study, recombinant canine CD3ε protein was generated via eukaryotic expression and used to immunize rabbits, yielding a novel rabbit anti-canine CD3ε monoclonal antibody (mAb), designated HORCF-CD3.1. Functional characterization confirmed its specificity through flow cytometry and Western blot, demonstrating robust binding to CD3 molecules. Furthermore, the mAb effectively induced T cell stimulation in vitro when applied as an anti-CD3 activator. These findings validate HORCF-CD3.1 as a versatile tool for ELISA, Western blot, and flow cytometry applications.
Discussion:
The successful development of this species-specific antibody provides a foundation for advancing diagnostic and therapeutic strategies targeting T cell-related diseases in both humans and dogs.
