High-Dimensional Single-Cell Analysis Reveals Coordinated Age-Dependent Neuroinflammatory Microglia-T cell Circuits...

Aging and cerebrovascular pathology drive neuroinflammation in vascular dementia (VaD) but immune mechanisms underlying this interplay remain unresolved. Leveraging multi-modal high-dimensional imaging, flow cytometry, and split pool ligation transcriptomic sequencing in a mouse model of VaD, we constructed a brain immune cell atlas spanning young and aged mice in health and disease. We profiled microglia, T cells, macrophages, neutrophils, and B cells and integrated transcriptomics, cell-cell communication, multiplex imaging, and comparative analysis with human microglia. We found striking depletion of Ccr7 ⁺ naïve T cells and expansion of Gzmk ⁺ cytotoxic Cd8 ⁺ effector memory T cells in the aging brain. At the same time, microglia shifted toward a pro-inflammatory state with enhanced activity of major histocompatibility class complex I (MHC-I) to T cell receptor and co-stimulation from CD86 to CD28. These shifts suggest enhanced neuroinflammatory polarization within the aged brain and in VaD. These signals were strongest from activated microglia to Gzmk ⁺ Cd8 ⁺ T_EM cells, indicating that age-related microglial polarization may sustain cytotoxic T cell activation in the aged brain. Our findings suggest pro-inflammatory microglia and Gzmk ⁺ CD8⁺ T_EM cells are central drivers of immune brain aging and highlights a therapeutic potential to disrupt age-related neuroinflammatory cascades in VaD.