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Complete genome sequence of Caulobacter vibrioides CB2A JS4038.
Beth Davenport1, Ryan McLaughlin2, Tony Liu3
1Department of Microbiology & Immunology, University of British Columbia, Vancouver, BC, Canada.
Microbiology Resource Announcements
|December 22, 2025
Summary
We sequenced the complete genome of Caulobacter vibrioides CB2A JS4038. This closed reference genome reveals genetic modifications for a novel plasmid-encoded S-layer display system.
Area of Science:
- Microbiology
- Genomics
- Molecular Biology
Background:
- Caulobacter vibrioides is a model organism for studying cell cycle and development.
- Strain CB2A JS4038 is a modified variant of C. vibrioides CB2A.
- Plasmid-encoded systems are crucial for introducing novel functionalities in bacteria.
Purpose of the Study:
- To obtain the complete genome sequence of Caulobacter vibrioides CB2A JS4038.
- To characterize the genetic modifications enabling a plasmid-encoded S-layer display system.
- To establish a closed reference genome for precise localization of genetic changes.
Main Methods:
- PacBio long-read sequencing technology for high-accuracy genome assembly.
- Bioinformatic analysis for genome annotation and variant identification.
- Comparative genomics to identify chromosomal alterations.
Main Results:
- A complete, closed genome sequence for Caulobacter vibrioides CB2A JS4038 was generated.
- Specific genotypic changes required for hosting the plasmid-encoded S-layer system were identified.
- The chromosomal locations of these genetic modifications were precisely determined.
Conclusions:
- The closed reference genome provides a valuable resource for Caulobacter vibrioides research.
- Understanding these genetic modifications facilitates the development of advanced S-layer display systems.
- This work advances the genetic engineering capabilities for Caulobacter species.
Keywords:
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