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Related Experiment Video

Updated: Jan 8, 2026

Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro
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Published on: December 5, 2025

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Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo

Sofia V Krylova1, Daniel Zamith-Miranda2, Alus M Xiaoli3

  • 1Department of Medicine, Albert Einstein College of Medicine; Fleischer Institute for Diabetes and Metabolism, Albert Einstein College of Medicine; Department of Molecular Pharmacology, Albert Einstein College of Medicine.

Journal of Visualized Experiments : Jove
|December 22, 2025
PubMed
Summary

Researchers developed a new mouse model to track adipocyte-derived extracellular vesicles (AdEVs) and their communication with adipose tissue cells. This reveals a novel paracrine signaling pathway within adipose tissue, important for metabolic health.

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Area of Science:

  • Endocrinology
  • Cell Biology
  • Extracellular Vesicles Research

Background:

  • Adipocytes function as endocrine cells secreting extracellular vesicles (EVs).
  • Mechanisms of EV secretion and their role in adipose tissue biology are not fully understood.

Purpose of the Study:

  • To develop tools for tracking adipocyte-derived EVs (AdEVs) in vivo and in vitro.
  • To investigate AdEV secretion dynamics and their interaction with adipose tissue cells.

Main Methods:

  • Generated a novel adipocyte-specific CD63-GFP reporter mouse line.
  • Utilized confocal imaging and flow cytometry to track AdEVs.
  • Established an in vitro system for monitoring AdEV secretion from differentiated adipocytes.

Main Results:

  • AdEVs are actively secreted and taken up by adipose tissue cells (APCs).
  • CD63-GFP signals indicated vesicle accumulation when secretion was inhibited.
  • A platform was established for biochemical and functional analysis of AdEVs.

Conclusions:

  • A robust genetic tool and in vitro system were developed to study AdEV secretion and targeting.
  • AdEVs mediate paracrine communication within the adipose microenvironment.
  • These findings provide a foundation for understanding AdEVs' role in metabolic health and disease.